TABLE 2.
Oligonucleotides used for gene cloning and P. aeruginosa mutation engineering
| Target gene | Oligonucleotide (5′→3′)a |
|---|---|
| Cloned | |
| flp | Flp11 Up (GAGGCAGGGCTCTGTCTCGGTGAT) |
| Flp12 Down (AAGCTTCTGAACGGCAGGCTTTGTAG) | |
| fppA | FppA Up-1 (TTAATACTAGTCGTCATTCGCTCCG) |
| FppA Down-1 (GTATTAGCCATAAGCATATAGTCG) | |
| fppA mutagenesis | FppA Up-2 (CAGGAAGATCGCTGTGAATTCGATG) |
| FppA Down-2 (GTGCATTGGAATGCAGCCATACCGAAT) | |
| fppA(D17A) | FppAD17A-Up (GCGGATATCAGGCCATCAAACGTT) |
| FppAD17A-Down (ACGTTTGATGGCCTGATATCCG) | |
| fppA(D78A) | FppAD78A-Up (GGCGCTGCCGCGGTCAAGTACCT) |
| FppAD78A-Down (AGGTACTTGACCGCGGCAGCGCC) | |
| Deleted | |
| flp | Flp1 (CGCACCGAGCAGCAGCAGGC) |
| Flp2 (CTCGCGTCACATTCTTGTTTGTTTGCTC) | |
| Flp3 (ACAAGAATGTGACGCGAGCGCTTTCCCGGT) | |
| Flp4 (TGCGGCTGAAGACGGGCTGT) | |
| tadA | TadA1 (GAGTCACCACCGCCGAACTCTATC) |
| TadA2 (CGCCGTTCACATAGCAGCGCCGCCTTGGCC) | |
| TadA3 (GCTGCTATGTGAACGGCGCGCAACTGCTGG) | |
| TadA4 (GGGTGAAGATGTCGCGCAGCGGCT) | |
| rcpA | RcpA1 (CGTCCTGCCCGGCGACTATGTCGA) |
| RcpA2 (CGCCTCCTACATATGCCGGCTCCCCTCAAGG) | |
| RcpA3 (CGGCATATGTAGGAGGCGCGCATGAACCAGAACT) | |
| RcpA4 (TGCTCCTGCAACGCCTTGGCCAG) | |
| fppA | FppA1 (CTCAGCACGGGGATGATCGC) |
| FppA2 (GTTTGCTCACATCCTGTTCTTCAGCCTTTTTT) | |
| FppA3 (AACAAGATGTGAGCAAACTTGCGGAAAAC) | |
| FppA4 (GCCATGCAGCAGTTCCTGCG) |
a
The mutagenic codons in FppAD17A-Up and FppAD78A-Up are indicated in bold.