FIG. 4.

Identification of the rep gene. The arrows indicate the pCW3-derived genes within the cloned insert of pJIR2768. Vertical lines with numbers represent independent EZ::TN insertion mutants within pJIR2768. Insertion derivatives are named by corresponding base pairs within the pCW3 sequence. A plus sign indicates that the EZ::TN-containing pJIR2768 derivative plasmid replicates independently in C. perfringens; a minus sign indicates that the EZ::TN-containing pJIR2768 derivative plasmid does not replicate independently in C. perfringens.