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Lack of association between MtrA and MtrC. Fluorescence polarization measured for FlAsH-labeled MtrC upon addition of indicated amounts of MtrA in the presence of 50 mM HEPES (pH 7.5), 140 mM KCl, and 2% OGP. Excitation was centered at 500 nm, and emission was centered at 528 nm. mP, milli-polarization units.
