TABLE 1.
Oligonucleotide primers for gene cloninga
| Name | Sequence |
|---|---|
| SO1779EF | CACCTAAGAAGGAGATATACATCCCATGATGAAACGGTTCAATTTC |
| SO1779C4ER | CTTGCAACAACCAGGGCAACATGCAGCGTTACCGTGTGCTTCCATC |
| SO1779EKER | AAGCTTGTCATCGTCATCGTTACCGTGTGCTTCCATCAATTG |
| SO1778EF | CACCTAAGAAGGAGATATACATCCCATGATGAACGCACAAAAATC |
| SO1778C4ER | CTTGCAACAACCAGGGCAACATGCAGCCATTTTCACTTTAGTGTGATCTG |
a
The sequences are from 5′ to 3′. The forward primers (EF) contain a stop codon (in italics) and a ribosome binding site (in boldface). The reverse primers (ER) contain a coding sequence for AACCPGCCK (C4) or DDDDKL (EK) (underlined).