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Interactions of purified MBP-ExpR1 and MBP-ExpR2 proteins with expR box DNA (A), an expR box with the second binding site (62-mer DNA fragment) (B), and a 62-mer DNA fragment (C) in the absence and presence of AHLs. DNA fragments were end labeled with [α-32P]dATP. Two nanograms of probe DNA was added to each reaction mixture. The amounts of proteins, unlabeled DNAs, 3-oxo-C6-HL, and 3-oxo-C8-HL used in each reaction are indicated above the gels.
