FIG. 4.

(A) Northern blot analysis of rsmA and Western blot analysis of RsmA. (B) Agarose plate assays for analysis of Peh, Prt, and Cel activities. (C) Northern blot analysis of exoenzyme genes. For the Northern blot analysis, each lane contained 10 μg of total RNA, and for the Western blot analysis each lane contained 10 μg of total protein. Equal loading of RNA was checked by hybridization of the blot with a probe corresponding to the 16S rRNA gene (rDNA). For exoenzyme assays, 50 μl of culture supernatant was loaded in each well. Enzymatic activities are indicated by halos around the wells on the assay plates. Column 1, AC5006 (AhlI⁺ ExpR1⁺ ExpR2⁺); column 2, AC5091 (AhlI⁻ ExpR1⁺ ExpR2⁺); column 3, AC5099 (AhlI⁻ ExpR1⁻ ExpR2⁺); column 4, AC5117 (AhlI⁻ ExpR1⁺ ExpR2⁻); column 5, AC5118 (AhlI⁻ ExpR1⁻ ExpR2⁻).