TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Erwinia carotovora subsp. atroseptica strains | ||
| Eca5 | Wild type | 50 |
| Eca12 | Wild type | 50 |
| Eca15 | Wild type | 50 |
| Eca20 | Wild type | 50 |
| Eca31 | Wild type | 50 |
| Erwinia carotovora subsp. betavasculorum Ecb168 | Wild type | J. E. Loper |
| Erwinia carotovora subsp. carotovora strains | ||
| Ecc71 | Wild type | 50 |
| AC5006 | Lac− derivative of Ecc71 | 35 |
| AC5091 | AhlI− derivative of AC5006 | 8 |
| AC5099 | AhlI− ExpR1− derivative of AC5006 | 12 |
| AC5115 | ExpR2− derivative of AC5006 | This study |
| AC5117 | AhlI− ExpR2− derivative of AC5006 | This study |
| AC5118 | AhlI− ExpR1− ExpR2− derivative of AC5006 | This study |
| Ecc193 | Wild type | 50 |
| AH2 | Wild type | A. K. Handa |
| SCC3193 | Wild type | 38 |
| SCRI193 | Wild type | 40 |
| EC153 | Wild type | 20 |
| Escherichia coli strains | ||
| DH5α | φ80lacZΔM15 Δ(lacZYA-argF)U169 hsdR17 recA1 endA1 thi-1 | Gibco BRL |
| MC4100 | araD139 Δ(lacIPOZYA)U169 recA1 thi-1 Strr | 26 |
| Plasmids | ||
| p34S-Gm | Gmr, source of Gmr cassette | 16 |
| pBluescript SK(+) | Apr | Stratagene |
| pCL1920 | Spr Smr | 25 |
| pLARF5 | Tcr | 22 |
| pMAL-c2g | Apr, protein expression vector | New England Biolabs |
| pMP220 | Tcr, promoter-probe vector | 43 |
| pRK415 | Tcr | 22 |
| pAKC781 | Apr, peh-1+ DNA in pBluescript SK(+) | 25 |
| pAKC783 | Apr, pel-1+ DNA in pBluescript SK(+) | 25 |
| pAKC882 | Apr, rsmA coding region in pT7-7 | 32 |
| pAKC936 | Spr, expR1+ in pCL1920 | 12 |
| pAKC938 | Spr, expR271+ in pCL1920 | This study |
| pAKC1034 | Apr, 200-bp celV fragment in pGEM-T Easy | 29 |
| pAKC1100 | Tcr, rsmA71-lacZ containing transcriptional start sites T1 and T2 in pMP220 | 6 |
| pAKC1102 | Tcr, rsmA71-lacZ containing transcriptional start site T1 in pMP220 | 7 |
| pAKC1103 | Tcr, rsmA71-lacZ containing transcriptional start site T2 in pMP220 | 7 |
| pAKC1106 | Tcr, rsmA153-lacZ, 0.4-kb rsmA153 upstream DNA in pMP220 | 7 |
| pAKC1223 | Tcr, expR2+ in pLARF5 from Ecc71 genomic library | This study |
| pAKC1224 | Apr, expR2 coding region in pMAL-c2g | This study |
| pAKC1225 | Apr, 2.0-kb BamHI-HindIII fragment containing expR2 from pAKC1223 cloned into pBluescript SK(+) | This study |
| pAKC1226 | Tcr, 2.0-kb BamHI-HindIII fragment containing expR2 from pAKC1223 cloned into pRK415 | This study |
| pAKC1227 | Tcr Gmr, expR2 derivative of pAKC1226 | This study |
| pAKC1228 | Spr, expR2153+ in pCL1920 | This study |
| pAKC1229 | Spr, expR2Eca15+ in pCL1920 | This study |
| pAKC1230 | Spr, expR2Eca20+ in pCL1920 | This study |
| pAKC1231 | Spr, expR2Ecb+ in pCL1920 | This study |
| pAKC1232 | Tcr, rsmAEca20-lacZ in pMP220 | This study |
| pAKC1233 | Tcr, rsmAEcb-lacZ in pMP220 | This study |