Figure 10.

Analysis of phosphorylation of p38 and ErbB2 at tyrosines 1248 and 1139, in the presence of the ErbB2 phosphorylation inhibitor AG825. (A) Immunoblot analyses of the phosphorylated forms of p38, ErbB2 pY1248, and pY1139 of CACO-2 cell lysates of Muc4-transfected cells treated with or without ErbB2 inhibitor AG825. A375 cell lysates were used as control. (B) Immunoblot of the activated form of p38 of CACO-2 lysates of Muc4 (lanes 1–3) and mock-transfected cells (lanes 4–6), under osmotic stress by treatment with 400 mM sorbitol. (C) X-z plane by confocal immunofluorescence of ErbB2 pY1248 and pY1139 in a confluent monolayer of CACO-2 cells in the presence and in the absence of AG825. First panel, Muc4 (green); second panel, staining with polyclonal anti-pY1248 or anti-pY1139; and third panel, merge of panels one and two.