Figure 10.

N-cadherin knockdown limits cell motility. (A) Mock infected, N-cadherin knockdown, and N-cadherin–overexpressing NMuMG/E10 cells were plated for 4 h on transwell filters coated in both the top and bottom sides with collagen I or fibronectin. Cells traversing the filter were photographed using a 10× objective (A) and quantified (B). The columns represent mean values and the bars represent the SD (∗p < 0.05, 1 versus 2; ∗∗p < 0.05, 5 versus 1 or 3). (C) NMuMG/E10 cells were plated on coated filters, and DMSO (vehicle), LY294002 (10 μM), or SP600125 (10 μM) was added to both the top and bottom chambers. NMuMG/E10 cells expressing Rac1N17 were plated on coated filters. The columns represent mean values and the bars represent the SD (∗p < 0.05, 1 versus 3, 5, or 7).