Figure 7.

RacN17 inhibits collagen I–induced morphological changes and N-cadherin up-regulation. (A) MOCK, RacN17, and RacV12 infected NMuMG/E10 cells were cultured for 2 d on noncoated (a, c, and e) or collagen I-coated (b, d, and f) dishes and photographed using a 10× objective. (B) Extracts (30 μg of protein) from cells cultured on noncoated or collagen I-coated dishes for 2 d were resolved by SDS-PAGE and immunoblotted for N-cadherin, E-cadherin, and tubulin (loading control). (C) N-cadherin mRNA levels were analyzed by quantitative real-time PCR as in Figure 3D (∗p < 0.05, col RacN17 versus col MOCK or col RacV12).