Figure 9.

N-cadherin knockdown prevents collagen I–induced changes. (A) Mock-infected, N-cadherin knockdown, and N-cadherin–overexpressing NMuMG/E10 cells were cultured on noncoated (a–c) or collagen I-coated (d–f) dishes. Photographs were taken of living cells using a 10× objective. (B) RIPA extracts (30 μg of protein) from mock-infected, N-cadherin knockdown, and N-cadherin–overexpressing NMuMG/E10 were resolved by SDS-PAGE and immunoblotted for N-cadherin, E-cadherin, and GAPDH. (C) Extracts (1 mg of protein) from cells cultured on noncoated or collagen I-coated dishes for 3 h were used for pull-down assay as in Figure 6A.