Table 2.
Plasmids used in this study
| Genotype | Name | Other name, description |
|---|---|---|
| pRS415 | LEU2 CEN (Sikorski and Hieter, 1989) | |
| pRS423 | HIS3 2μ (Christianson et al., 1992) | |
| pCEN-HIS3-LEU2-MET15 | pCK283 | pMRT2; HIS3 and MET15 genes inserted in pRS415 |
| pPGAP1-lacZ | pMS29 | PGAP1-lacZ fusion at codon 53 of GAP1; CEN (Stanbrough and Magasanik, 1995) |
| pGAP1-GFP | pCK230 | pSH40; GAP1-sGFP fusion under the GAP1 promoter; CEN (Helliwell et al., 2001) |
| pPCUP1-myc-UBI | pCK322 | pMRT7; pRS306 2μ containing PCUP1-myc-UBI expression cassette |
| pPCUP1-UBI | pCK331 | pMRT15; pRS306 2μ containing PCUP1-UBI expression cassette |
| pBUL1 | pCK323 | pSH53; pRS423 containing BUL1 ORF plus 5′ and 3′ regions |