Figure 1.

Expression of Cas mRNA in developing mouse cerebella. (A) Semiquantitative RT-PCR analysis of Cas mRNA expression in P7 or P21 mouse tissue. (B) RT-PCR analysis of Cas mRNA expression in postnatal cerebella at six different postnatal stages. RT-PCR for GAPDH was used as an internal control. (C–H) In situ hybridization analysis of Cas distribution in the P7 (C, D, and G) and P21 (E, F, and H) mouse brains. C, P7 brain; D, P7 cerebellum; E, P21 brain; F, P21 cerebellum; G, P7 cerebellar cortex; H, P21 cerebellar cortex. EGL, external granule cell layer; ML, molecular layer; IGL, internal granule cell layer; PL, Purkinje cell layer; WM, white matter.