Figure 3.

Cas is enriched in the growth cones of cerebellar granule cells. (A) Immunoblotting of Cas in subcellular protein fractions from P7 and P21 mouse cerebella. (B) Immunoblotting of Cas and YP-Cas in growth cone fractions from P7 mouse cerebella. Equal amounts of proteins were loaded in each lane and immunoblotted with antibodies against YP-Cas, Cas, and GAP43 (as a control of growth cone proteins). (C–E) Confocal images of Cas (C) and Map2 (D) in the granule cells (DIV1). (E) Merged image of C and D. (F–I) Phase-contrast images (F) of Cas (G) and Map2 (H) in the granule cell growth cones. (I) Merged image of G and H. (J–L′), Immunostaining of Cas (J) and F-actin (by phalloidin staining; K) in granule cells. (L) Merged image of J and K. (L′) Magnified views of the growth cones. Arrows indicate colocalization of Cas and F-actin.