Table 2.

Distribution of GFP fluorescence

Promoter	GFP-fusion	Small-budded cellsa			Large-budded cellsa
		b < m (%)	b = m (%)	b > m (%)	b < m (%)	b = m (%)	b > m (%)
TIP1	Tip1p	100	0	0	98.5	1b	0.5b
	Cwp2p	100	0	0	98	2b	0
	Cwp1p	100	0	0	99	1b	0
CWP2	Cwp2p	19	31	50	14	71	15
	Tip1p	40	25	35	21	67	12
CWP1	Cwp2p	30	21	49	8	67	25
	Tip1p	32	16	52	11	57	32

^a From cultures of cells expressing each construct (see Figures 1 and 2), we counted ≥100 budded cells with bud diameter <50% that of the mother cell (small-budded cells) and ≥100 budded cells with bud diameter >50% that of the mother (large-budded cells). Bud wall fluorescence intensity was scored as lower than (b < m), equal to (b = m), or greater than (b > m) that of the mother.

^b Examination of the CFW staining suggested that each of these cells had a septum and thus that each was actually a pair of unbudded cells.