Figure 2.

Deletion of LAA1 enhances the α-factor processing defect of chc1-ts and GGA deletions. The same strains as in Figure 1, plus gga2Δ (GPY2149), gga2Δ laa1Δ (GPY2957), and gga2Δ apl2Δ (GPY2420-3C) strains, were metabolically labeled with [³⁵S]methionine for 45 min at 24°C. The α-factor was immunoprecipitated from the culture supernatants and analyzed by SDS-PAGE and autoradiography. All samples were run on the same gel, and lanes were rearranged for presentation. Unprocessed α-factor was quantified by phosphorimaging analysis.