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Laa1p colocalization with clathrin, AP-1, and Gga2p. Cells expressing GFP-tagged Laa1p (Laa1-GFP) together with mRFP-tagged clathrin heavy chain (GPY3028, Chc1-mRFP) (A), AP-1 β1 subunit (GPY3034, Apl2-mRFP) (B), or Gga2p (GPY3018, Gga2-mRFP) (C) were grown to logarithmic phase and visualized by epifluorescence microscopy. (D) Cells expressing Gga2-mRFP and GFP-tagged β1 (Apl2-GFP) together (GPY3109) were visualized as described in A–C.
