Figure 1.
Annexin A4 localization and translocation in living cells. (A) EYFP-annexin A4 expressed in HeLa, N1E-115, and MDCK2 cells translocated to cell membranes upon Ca2+ elevation with ionomycin. The onset and duration of translocation was variable between experiments. In some cases protein translocation started immediately and in others with a delay of up to 5 min. (B) The core of annexin A4 behaved like wild-type protein in HeLa cells. Graphs show protein distribution across cells. White bars indicate position of the measurement. (C) Sequence of images showing annexin A4 translocation in the cytosol and delayed translocation in the nucleus of N1E-115 cells (from Supplementary Video 1). Images were taken every 10 s. Ionomycin was added after 2.5 min.