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. 2006 Jul;17(7):3318–3328. doi: 10.1091/mbc.E06-01-0041

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© 2006 by The American Society for Cell Biology

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Figure 8. — Annexin A4 effect on membrane protein mobility. (A) Photobleaching of CHRM2-EYFP in HeLa cells. (B) Photobleaching of CHRM2-EYFP in HeLa cells coexpressing ECFP-annexin A4 after ionomycin-induced annexin A4 translocation. (C) Representative traces of CHRM2-EYFP fluorescence recovery in the bleached region of the HeLa cells expressing only the receptor, or both the receptor and annexin A4 (translocated to the membrane using ionomycin), as in A and B. (D) Average recovery after photobleaching of four different membrane proteins (CHRM2-EYFP, ErbB1-EYFP, EYFP-Mem, and GPI-EYFP), expressed alone or together with ECFP-annexin A4 in HeLa cells, with or without ionomycin treatment. Error bars, SD of 3–5 experiments. (E) Average recovery after photobleaching of ErbB1-EYFP expressed alone or together with ECFP-annexin A4 in N1E-115 cells, with or without ionomycin treatment. Error bars, SD of 4–9 experiments.