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Titin immunoglobulin domain I27 (TI27). I27 is a β-sandwich with four β-strands on each sheet. Hydrogen bonds between the N-terminal β-strand A′ and the C-terminal strand G are shown. The simulations are carried out by moving the restraint point (blue) to the right by fixed distances followed by MD calculations. Simulations are carried out for values of the spring constant k ranging from 0.5 to 5.0 kcal/mol Å2.
