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Polarized FTIR spectra of reconstituted nAChR PC/PA/Chol 3:1:1 membrane films. FTIR spectra collected at 22.5°C with perpendicular polarized light (dashed lines) and parallel polarized light (solid lines) are illustrated. The FTIR spectra recorded with perpendicular polarized light were multiplied by a factor of 2 to account for differences in the evanescent field strength (RISO).
