FIGURE 5.

Carb-difference spectra recorded from the nAChR in PC/PA/Chol 3:1:1 membranes. (A) Carb-difference spectra recorded with unpolarized infrared light. The top trace was recorded in ¹H₂O buffer, whereas the middle trace was recorded in ²H₂O buffer. The bottom trace is a control showing the difference between spectra both recorded in the absence of Carb. (B) Carb-difference spectra recorded with parallel (top trace and solid line in bottom traces) and perpendicular (middle trace and dashed line in bottom traces) polarized infrared light. The perpendicular polarized difference spectrum has been scaled to account for the difference in evanescent field strength (R_ISO).