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Domains identified by the HCCP method in the dipeptide-binding protein from E. coli (PDB codes 1DPP for the closed form and 1DPE for the open form). The loop containing residues 408–411 is marked by the dashed oval. (a) Open form. (b) Closed form without ISE (the loop belongs to the “dark” domain). (c) Closed form with ISE (the loop belongs to the “light” domain).
