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. 2006 Apr 28;91(2):744–758. doi: 10.1529/biophysj.105.073312

FIGURE 3.

FIGURE 3

Specificity of the extension and force measurements. (A) BoNT-B in the presence of zinc ions (Zn2+) cleaves recombinant Sb2 as revealed by the reduction in Sb2 immunoreactivity on functionalized coverslips (B, +) and by the reduction of Sb2 immunoreactive band on Western blots (C, +) when compared to their controls (B and C, −). Dashed box in A indicates epitope recognized by anti-Sb2 antibody (for details see “Materials and Methods”). The drawing in A is not to scale. Scale bar in B, 10 μm.