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. 2006 Apr 28;91(2):744–758. doi: 10.1529/biophysj.105.073312

FIGURE 8.

FIGURE 8

The force and extension values for dissociation of SNARE proteins as a function of the force loading rate. (A) The force necessary to take apart the Sx1A-Sb2 in the absence (circles) or presence of SNAP25B (squares) increases exponentially with the increase in the loading rate. (B) The extension changes significantly with the loading rate only when Sx1A-Sb2 interactions are ruptured, but not when SNAP25B is present with Sx1A-Sb2. Points represent mean ± SE (61–100 events in A and 33–272 events in B). The dashed lines indicate fits to the data described by either an equation force = a + b × ln (force loading rate) in A, where a = −373 pN and −96 pN, whereas b = 63 pN and 34 pN, for Sx1A-Sb2 (r = 0.9), and Sx1A-Sb2-SNAP25 (r = 0.84) interactions, respectively, or an equation extension = a + b × ln (force loading rate) in B, where a = −12.0 nm and b = 3.4 nm for Sx1A-Sb2 interactions (r = 0.89). The force loading rate is in pN/s. The solid line indicates that the extension value is constant at 11.6 nm as it does not change with the loading rate when measuring the Sx1A-Sb2-SNAP25B interactions.