TABLE 2.
Specific interactions between syntaxin 1A and synaptobrevin 2
| Tip | Coverslip | Positive | Tested | Positive (%) |
|---|---|---|---|---|
| Ni2+ | Ni2+ | 0 | 350 | 0 |
| Sx1A-H6 | Sb2-H6 | 2146 | 5652 | 38 |
| H6 | Sb2-H6 | 1 | 200 | <1 |
| Sx1A-H6 | H6 | 2 | 320 | <1 |
| Sx1A-H6 | Sb2-H6 | 65 | 182 | 36 |
| Sx1A-H6 + GST-Sb2 | Sb2-H6 | 71 | 1482 | 5 |
| Sx1A-H6 + GST | Sb2-H6 | 194 | 494 | 39 |
| Sx1A-H6 | Sb2-H6 | 105 | 286 | 37 |
| Sx1A-H6 | Sb2-H6 + GST-Sx1A | 72 | 1378 | 5 |
| Sx1A-H6 | Sb2-H6 + GST | 174 | 494 | 35 |
| Sx1A-H6 | Sb2-H6 | 341 | 1010 | 34 |
| Sx1A-H6 + Sb2-H6 | Sb2-H6 | 14 | 500 | 3 |
| Sx1A-H6 | Sb2-H6 + Sx1A-H6 | 21 | 494 | 4 |
Note: specific interactions refer to the def segment of the force-distance curves (see Figs. 1 E and 2 A); retraction velocity is 1.6 μm/s; spaces separate matching sets of experiments.
Abbreviations: GST, glutathione S-transferase; H6, six consecutive histidines tag; Sb2, synaptobrevin 2; Sx1A, syntaxin 1A.