Figure 6.

Accumulation of c-myc mRNA in dog thyrocytes analyzed by Northern blotting. Quiescent 4-day-old cells were stimulated for 1 or 3 h with TSH (T), EGF+serum (ES) with or without TGFβ or by TGFβ (β) alone or remained in control (C) condition. Northern blots were prepared with 10 μg of glyoxal denatured total RNA. Acridine orange was performed to assess that equal amount of RNA were loaded in independent lanes.