Skip to main content
. 2006 Jul 3;116(7):1924–1934. doi: 10.1172/JCI27597

Figure 8. Neurogenesis but not oligodendrocyte generation is affected by PAC1 overexpression.

Figure 8

(A and B) Double immunostaining of E15.5 cortex with BrdU (red) and GLAST (green) showed reduced BrdU labeling in the subventricular zone (SVZ) of developing cortex of Tg embryos (B) compared with that in the WT control (A). Note that most, if not all, of the BrdU-labeled cells coexpressed GLAST. (C and D) Colabeling of E15.5 cortex with BrdU and PDGFRα. Cells in the ventricular zone (VZ) and SVZ at this stage were weakly stained with anti-PDGFRα, indicating they are multipotential neural stem cells. No significant difference in PDGFRα staining was observed at E15.5 in cortex of WT and Tg embryos. (E and F) Immunostaining of Sox10 showed comparable labeling of Sox10-positive cells in P12 striatum of WT (E) and Tg (F) littermates. Scale bars: 50 μm in A–F.