Figure 9. PACAP promotes neurite outgrowth and migration in neurons of Tg embryos.

At E15.5, neurons from developing cortex of Tg or WT embryos were cultured on 35-mm dishes at a density of 10⁴ cells/ml in the absence (A and C) or presence (B and D) of PACAP (10^–7 M) for 4 hours prior to recording. Migrational behavior was recorded with time-lapse imaging for the subsequent 8 hours, and migration rates of 100–120 cells under each condition from 3 independent experiments were quantified by 1-way ANOVA (*P <0.05) (E). Note that (a) PACAP promotes neurite outgrowth in neurons of both WT and Tg embryos (B and D) and (b) PACAP significantly increases the migration rate of neurons of Tg embryos (E).