Table 1.
Crystallographic statistics
| Resolvase:Hin chimera | Activated apo resolvase | |
|---|---|---|
| Spacegroup | P21212 | P212121 |
| Resolution, Å | 50.0–3.5 | 50–2.1 |
| Rmerge,* % | 9.1 | 8.9 |
| I/σ† | 12.9 (1.2) | 15.7 (1.9) |
| Completeness, % | 96.5 (84.3) | 99.8 (100) |
| Unique reflections | 16,189 | 59,517 |
| Redundancy | 3.5 | 3.8 |
| Copies in AU | 2 | 4 |
| rms deviation bond length, Å | 0.008 | 0.007 |
| rms deviation bond angle, ° | 1.2 | 1.0 |
| Rcryst,‡ % | 28.2 (20–3.5 Å) | 21.9 |
| Rfree,‡ % | 32.3 (20–3.5 Å) | 25.5 |
| PDB ID | 2GM4 | 2GM5 |
*Rmerge is Σj|Ij−〈I〉|, where Ij is the intensity of an individual reflection and 〈I〉 is the mean intensity for multiply recorded reflections.
†I/σ is the mean of intensity divided by the SD. The values in brackets are for the high resolution shell (3.63–3.5 Å and 2.18–2.1 Å, respectively).
‡Rcryst is Σ‖Fo|−|Fc‖/Σ|Fo|, where Fo is an observed amplitude and Fc is a calculated amplitude; Rfree is the same statistic calculated over a subset of the data that have not been used for refinement.