Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Jun 13;34(10):3169–3180. doi: 10.1093/nar/gkl407

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2006 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Analysis of crosslinking SC-MutH^A223C to its activator protein MutL by SDS–PAGE. (A) Photocrosslink reactions of the heterocomplex formed by SC-MutH^A223C modified with MBP and the indicated MutL variants. After complex formation with MutL and UV-irradiation new bands are observed (H–L). Note that both MutL^R531A and MutL^R531E fail to form a photocrosslink with SC-MutH^A223C. (B) Chemical crosslinking of SC-MutH^A223C to the indicated MutL variants with the cysteine specific homobifunctional crosslinker BM[PEO]₄. All MutL variants but MutL^CF are able to form a chemical crosslink with SC-MutH^A223C.