Table 4.

Change value of R_ct of each step before and after treatment of 100 mM EDTA and change ratio of R_ct after binding GT heteroduplex

	HS-NTA	Ni2+	MutS	GT 2 µM
Experiment 1 Rct (Ω)	(1.43 ± 0.10) × 104	(9.09 ± 0.08) × 103	(2.43 ± 0.51) × 104 %(ΔRct): 33%	(3.24 ± 0.23) × 104
Treatment of 100 mM EDTA for 4 h
Experiment 2 Rct (Ω)	(1.09 ± 0.23) × 104	(7.95 ± 0.07) × 103	(2.89 ± 0.47) × 104 %(ΔRct): 35%	(3.90 ± 0.32) × 104

After treatment of EDTA on the GT heteroduplex modified electrode, nearly same value of the change ratio of R_ct was appeared. Values in the table are the mean ± SD of three repeated experiments.