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. 2000 Mar;11(3):1093–1101. doi: 10.1091/mbc.11.3.1093

Figure 3.

Figure 3

Polarized transport of C6-NBD–SM depends on the degree of cell polarity. The SAC was loaded with C6-NBD–SM as described in MATERIALS AND METHODS. Transport of the lipid analogue from the SAC was then monitored for 20 min in back exchange medium. In (A), the percentage of C6-NBD-SM–labeled BCP was determined in 18- (□) and 72-h-old (░⃞) cell cultures. The asterisk marks the statistically significant (Student's t test, p < 0.05) decrease in BCP labeling in 72-h-old cell cultures, when compared with the 18-h-old cell cultures. In (B) and (C), the distribution of the lipid analogue within the labeled BCP of 18- and 72-h-old cell cultures, respectively, was analyzed: the compartmental distribution of the lipid is shown before (□) and after ░⃞ a 20-min chase from the SAC. *Statistically significant differences (Student's t test, p < 0.05) between the corresponding BCP compartments (BC, SAC, and BC+SAC) in (B) and (C).