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Antiapoptotic action of ΔRaf-1:ER after Rho inhibition. CCL39–ΔRaf-1:ER monolayers were treated with 60 pM toxin B (ToxB) in serum-free medium in the presence (+) or absence of 1 μM estradiol. (A) Polymerized actin was detected using phalloidin-FITC, and nuclei were labeled with DAPI. Magnification: 630×. (B) Western blot analyses of PARP cleavage (top) and MAP kinase activation (bottom) were performed on total cell lysates.
