Figure 2.

Serum removal potentiates induction of apoptosis. Exponentially growing CCL39 cells were deprived of serum (top) or anchorage and serum (bottom) as described in MATERIALS AND METHODS. At the indicated times, attached cells were trypsinized and treated as suspended cells. Annexin V-FITC labeling, TUNEL analyses, nuclei staining with DAPI, and immunoblot analysis of PARP were performed as described in Figure 1. Magnification: 400×.