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Z-Val-Ala-d,l-Asp-fluoromethylketone blocks apoptosis induced by anchorage and serum removal. Z-Val-Ala-d,l-Asp-fluoromethylketone (ZVAD) was added at the time of anchorage and serum removal. Immunoblot analysis of PARP was performed on total cell lysates from cells in suspension for the indicated times.
