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Raf activation in CCL39–ΔRaf-1:ER cells stimulates the MAP kinase pathway without activation of the PI3-K/Akt pathway. CCL39–ΔRaf-1:ER monolayers arrested in G0 by 18 h of serum removal were stimulated with estradiol or insulin for the indicated times. Western blot analyses were performed on whole-cell lysates using antibodies that recognize Akt or MAP kinase or their phosphorylated forms. p-Akt, phospho-Akt.
