Figure 6.

Inhibition of apoptosis after anchorage and serum withdrawal by Raf-1/MAP kinase activation. CCL39–ΔRaf-1:ER cells were deprived of anchorage and serum in the presence or absence of 1 μM estradiol (Est) for the indicated times. (A) Phase-contrast photomicrographs (magnification: 200×) of cells taken after 12 h (left) and propidium iodide staining (right) revealing dead, permeable cells. (B) Time course of PARP cleavage (top) and MAP kinase activation (bottom) after the detachment of cells in the presence or absence of estradiol. (C) Dose response of estradiol-induced inhibition of PARP cleavage (top) and activation of MAP kinase (bottom) after 12 h of anchorage and serum deprivation.