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. 2000 Mar;11(3):1103–1112. doi: 10.1091/mbc.11.3.1103

Figure 7.

Figure 7

Effect of MEK-1 inhibition or stimulation on the cleavage of PARP. (A) Western blot analyses of PARP cleavage (top) and MAP kinase activation (bottom) were performed on total cell lysates from CCL39–ΔRaf-1:ER cells deprived of anchorage and serum for 12 h in the presence of 0.1 μM estradiol and 20 μM PD98059, as indicated (+). (B) Nontransfected cells (parental) or cells stably expressing constitutively active MEK-1 (MEK SS/DD) were deprived of anchorage and serum. At the indicated times, PARP cleavage (top) and MAP kinase activation (bottom) were analyzed by Western blotting.