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Effect of MAP kinase and PI3-K inhibition on PARP cleavage. Western blot analyses of PARP cleavage (top) and MAP kinase activation (bottom) were performed on total cell lysates from CCL39–ΔRaf-1:ER cells after 20 h of suspension culture in serum-supplemented medium in the presence (+) or absence of 20 μM PD98059 or 15 μM LY294002.
