Figure 1.

Overexpression of calmodulin and distinct calmodulin mutant alleles restores endocytosis in arc35-1. (A) Wt (RH2877) and arc35-1 (RH3429) strains without plasmid or bearing plasmid pCMD1 (+Cmd1p) were assayed for their ability to internalize radioactive α-factor at 24 and 37°C after a preincubation of 20 min at the indicated temperature. (B) Wt (RH2877), arc35-1 (RH3429), and arp2-1 (RH4164) strains without plasmid or overexpressing the indicated mutant calmodulin protein from pASZ12 were assayed for the internalization of radioactive α-factor at 24 or 37°C after a preincubation of 20 min. The results are presented as the percentage of radioactive α-factor internalized per minute during the linear range of the uptake reaction.