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. 2000 Apr;11(4):1113–1127. doi: 10.1091/mbc.11.4.1113

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Copyright © 2000, The American Society for Cell Biology

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Suppression of arc35-1 ts growth by calmodulin. Wt (RH2877) (A), arc35-1 (RH3429) (B), and arp2-1 (RH4164) (C) strains were transformed with the following plasmids: (A) pASZ12, (B) pCMD1, (C) pASZ12::cmd1-226 (actin), (D) pASZ12::cmd1-228 (calmodulin mislocalized), (E) pASZ12::cmd1-239 (nuclear defect), (F) pASZ12::cmd1-231 (bud emergence), (G) pASZ12::cmd1-247 (endocytosis), (H) pASZ12::cmd1-3 (calcium binding). The strains were grown on glucose-selective medium for 3 d at 24°C and for 2 d at 37°C.