Figure 3.

Effect of activated Gα_o on EGF receptor tyrosine phosphorylation. CHO cells in 10-cm dishes were transfected with 5 μg of EGFR and 5 μg of empty vector, Gα_o-WT, or Gα_o-Q205L, serum starved for 18 h, and stimulated with 100 ng/ml EGF for 5 min. Immunoprecipitation (IP) with anti-EGFR antibodies and immunoblot analysis of the immunoprecipitates with anti-phosphotyrosine or anti-EGFR antibodies were performed as described in MATERIALS AND METHODS. The molecular masses (kilodaltons) of marker proteins are shown to the left, and the position of the EGFR is indicated by arrows. Data are representative of three similar experiments.