Figure 6.
Activated Gαo does not affect MAPK stimulation by FGF receptors. (A) Effect of FGF on Raf activity in nontransfected CHO cells grown in 10-cm dishes. (B) Effect of Gαo on FGF-stimulated MAPK activity in CHO cells transfected in 6-cm dishes with 1.4 μg of HA-p44 MAPK and 1.4 μg of empty vector, Gαo-WT, or Gαo-Q205L. Cells were serum starved for 18 h before stimulation with 40 ng/ml FGF for 5 min. Raf-1, A-Raf, B-Raf, and HA-p44 MAPK activities were determined as described in the legends to Figures 1 and 5. Data are means ± SE for duplicate samples from two independent experiments.