Figure 1.

Amino acid sequence of the human helicase-type protein NOH61 and comparison with its putative yeast homologue Dbp9p. (A) Schematic representation of highly conserved amino acid regions characteristic of putative ATP-dependent RNA helicases of the DEAD-box protein family (Schmid and Linder, 1992; Pause and Sonenberg, 1993). The nine conserved motifs are boxed (X indicates any amino acid). (B) Amino acid sequence of the protein NOH61 deduced from the cDNA insert of pBT-NOH61 (EMBL accession number AJ131712, 1926 bp). The helicase core region extends from aa 51 to 378. The nine conserved motifs characteristic of the family of DEAD-box proteins are all present in the primary sequence of NOH61 (indicated by bold letters). Putative nuclear localization signals are double underlined, and sequences used for generating antibodies are marked by dotted lines. (C) Sequence comparison of human protein NOH61 with the yeast protein Dbp9p (EMBL accession number Q06218). Horizontal bars are omissions introduced to optimize the alignment. The symbols below the primary sequences are defined as follows: *, identical amino acids; 1–5, conservative exchanges (1, aliphatic, polar; 2, acidic; 3, basic; 4, aliphatic, nonpolar; 5, aromatic).