Figure 4.

RT-PCR analysis of Gipi3k1 and Gipi3k2. Purified RNA extracted from G. intestinalis trophozoites (T) and 36 hr encysting cells (En) was converted to cDNA via a reverse transcriptase (RT) reaction and subjected to PCR to amplify regions encoding the apparent insertions in GiPI3K1 and GiPI3K2. The resulting PCR products were separated on an agarose gel. Minus RT controls were also carried out to ensure product originated from RNA only (not shown). Molecular size markers are in kilobases.