Figure 1.
Amoebicidal effect of human and bovine milk. 1x106 trophozoites were placed in medium BI-S-33 (A) or in low-iron medium (B), each one containing the percentage of human and bovine milk indicated. (C) Similar to (B), but none or 20% of porcine milk was used, and 1 mg/ml of bovine or human apo-lactoferrin were added together with porcine milk. In all cases, the cultures were incubated for 1 hour at 37°C and then cells were washed and stained with propidium iodide and trypan blue to determine viability. Experiments were done three times in triplicate. The mean and SD were evaluated.