Figure 1.

Mpr1 is a fission yeast homologue of the budding yeast Ypd1 protein, a response regulator phosphotransferase. (A) Alignment of the amino acid sequence of Mpr1 with that of Ypd1. Identical residues are shown by shaded boxes. An asterisk marks the phosphorylation site His-64 of Ypd1, which is also conserved in Mpr1. The nucleotide sequence of mpr1⁺ is available in the GenBank database under accession number AL034352 (ORF, SPBC725.02). (B) Restriction map of the mpr1⁺ locus and the mpr1::his7⁺ construct for gene disruption. The HindIII–XhoI fragment encoding residues 136–281 was replaced with the his7⁺ marker gene (Apolinario et al., 1993) to disrupt the mpr1⁺ gene. Restriction enzyme sites: Hd, HindIII; RV, EcoRV; Sl, SalI; Xh, XhoI.