Figure 2.

GPI8 protein sequence of L. mexicana (Lm; this study) aligned with S. cerevisiae (Sc; SwissProt, accession number P39012), human (Hs; Q92643), and C. ensiformis legumain (Leg; P49046). Gaps (.) were introduced to help the alignment; the C-terminal ends of Sc, Hs, and Leg have been omitted. Amino acid identities and similarities are highlighted. The highly conserved regions used to design degenerate oligonucleotide primers to isolate the L. mexicana GPI8 are overlined and numbered. Conserved cysteine (∗), histidine (#) and serine (^) residues are indicated. L. mexicana GPI8 has three potential N-glycosylation sites at positions N¹⁶³, N²⁶⁸, and N³⁰⁷, none of which is conserved with yeast GPI8 that is heavily glycosylated (Benghezal et al., 1996).