Table 2.

Dependency of nuclear migration success on nuclear positioning before anaphase B in wild-type and cnm67Δ cells

The relation between nuclear positioning and subsequent nuclear migration success was analyzed by time-lapse fluorescence microscopy of diploid Hhf2-GFP–labeled cells. In all cases the last time point before nuclear elongation was taken into account for the depicted nuclear positioning. Nuclear migration was counted as failed when the nucleus had not entered the bud at the time the cell started a new cell cycle with new bud emergence. Later entry of a nucleus into a bud from a previous cell cycle was not observed. (wild-type, n = 120; cnm67Δ, n = 325).